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KMID : 0370219870310020105
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Yakhak Hoeji
1987 Volume.31 No. 2 p.105 ~ p.111
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HPLC Determination of Carboxyl Group Using 2-Bromoacetyltriphenylene as a Pre-labeling Reagent Separative determination of bile acids by HPLC
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박만기/Park MK
정해수/양호길/Chung HS/Yang HG
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Abstract
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A high performance liquid chromatographic method was developed for the simultaneous determination of free and glycine conjugated bile acids. Free and glycine conjugated bile acids were extracted from bear gall bladder by methanol and from serum using a Sep-pak C18 catridge. The extracted bile acids were labeled with 2-bromoacetyltriphenylene in acetonitrile using 18-crown-6-ether as a catalyst. Derivatized bile acids were separated from the individual bile acids on a reversed-phase column (Chemcosorb 5-ODS-H) using acetonitrile-methanol-water(10:50:30) as a mobile phase and monitored by an UV-detector at 254nm. Linearities of calibration curve were obtained between 4ng and 24ng, and recoveries from bear gall bladder sample were higher than 94%.
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KEYWORD
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